Betreff: Fruit flies....cell phone radiation....reproductive damage....2006
Datum: Wed, 15 Nov 2006 10:46:21 EST


Cell death induced by GSM 900-MHz and DCS 1800-MHz mobile telephony radiation

Dimitris J. PanagopoulosCorresponding Author Contact Information, a, E-mail The Corresponding Author, Evangelia D. Chavdoulaa, Ioannis P. Nezisa and Lukas H. Margaritisa

aDepartment of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Panepistimiopolis, 15784 Athens, Greece

Received 21 April 2006;  revised 8 August 2006;  accepted 28 August 2006.  Available online 11 October 2006.    -    MUTATION RESEARCH/GENETIC TOXICOLOGY & ENVIRONMENTAL MUTAGENESIS


In the present study, the TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay – a well known technique widely used for detecting fragmented DNA in various types of cells – was used to detect cell death (DNA fragmentation) in a biological model, the early and mid stages of oogenesis of the insect Drosophila melanogaster. The flies were exposed in vivo to either GSM 900-MHz (Global System for Mobile telecommunications) or DCS 1800-MHz (Digital Cellular System) radiation from a common digital mobile phone, for few minutes per day during the first 6 days of their adult life. The exposure conditions were similar to those to which a mobile phone user is exposed, and were determined according to previous studies of ours [D.J. Panagopoulos, A. Karabarbounis, L.H. Margaritis, Effect of GSM 900-MHz mobile phone radiation on the reproductive capacity of D. melanogaster, Electromagn. Biol. Med. 23 (1) (2004) 29–43; D.J. Panagopoulos, N. Messini, A. Karabarbounis, A.L. Philippetis, L.H. Margaritis, Radio frequency electromagnetic radiation within “safety levels” alters the physiological function of insects, in: P. Kostarakis, P. Stavroulakis (Eds.), Proceedings of the Millennium International Workshop on Biological Effects of Electromagnetic Fields, Heraklion, Crete, Greece, October 17–20, 2000, pp. 169–175, ISBN: 960-86733-0-5; D.J. Panagopoulos, L.H. Margaritis, Effects of electromagnetic fields on the reproductive capacity of D. melanogaster, in: P. Stavroulakis (Ed.), Biological Effects of Electromagnetic Fields, Springer, 2003, pp. 545–578], which had shown a large decrease in the oviposition of the same insect caused by GSM radiation. Our present results suggest that the decrease in oviposition previously reported, is due to degeneration of large numbers of egg chambers after DNA fragmentation of their constituent cells, induced by both types of mobile telephony radiation. Induced cell death is recorded for the first time, in all types of cells constituting an egg chamber (follicle cells, nurse cells and the oocyte) and in all stages of the early and mid-oogenesis, from germarium to stage 10, during which programmed cell death does not physiologically occur. Germarium and stages 7–8 were found to be the most sensitive developmental stages also in response to electromagnetic stress induced by the GSM and DCS fields and, moreover, germarium was found to be even more sensitive than stages 7–8.

Keywords: Mobile telephony radiation; RF; GSM; DCS; Cell death; DNA fragmentation; Electromagnetic fields; Drosophila; Oogenesis

Corresponding Author Contact InformationCorresponding author. Tel.: +30 210 7274273; fax: +30 210 7274742.

Fruit flies exposed to cell phone radiation for 6 minutes per day over 6 days had reproductive damage. The experiments used actual cell phones activated with someone speaking throughout the exposure. Both GSM 900 and DCS 1800 mobile telephony radiations strongly induce cell death (DNA fragmentation) in ovarian egg chambers of the exposed groups. Insects typically are thought more resistant to radiation than people. Mutation Research More... [related stories]